Serodiagnosis of Oesophagostomum Columbianum infection in Goats using indirect ELISA
نویسندگان
چکیده
منابع مشابه
Diagnosis of mixed gastrointestinal nematode infection in goat by an indirect-ELISA
An indirect-ELISA for the diagnosis of mixed gastrointestinal (GI) nematode infection comprising Oesophagostomum, Haemonchus and Trichuris species was standardized using crude somatic antigen of Oesophagostomum columbianum (CSAg-Oc) and sera of slaughtered goats with known parasitological status including Oesophagostomum, Haemonchus, and <em...
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In the present investigation, E/S antigen from O. columbianum was isolated by in vitro culture method and hyper immune serum was raised against this antigen in rabbits. On SDS – PAGE analysis, characterization, the E/S antigen revealed six polypeptides at 29.0, 38.0, 49.0, 71.0, 84.0 and 95.0 kDa. Further, three immuno-reactive polypeptides at 29.0, 38.0 and 49.0 kDa were detected in western bl...
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Melioidosis caused by gram negative bacteria, B. pseudomallei, is a fatal disease in the tropical and sub-tropical regions. However, sporadic cases have been reported in elsewhere. Early detection is imperative to reduce the mortality rate. Serological tests have being substantially developed using recombinant proteins as specific targeted antigens to melioidosis antibodies. In the present stud...
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متن کاملIn vitro study of factors related to the hatching of eggs of Oesophagostomum columbianum
To determine the in vitro effects of various environmental and nutritional factors on the hatching of eggs, development and survival of larvae of Oesophagostomum columbianum, eggs were cultivated at different temperature gradients, humidity and P in phosphate buffer saline (PBS), tap water (TW) and normal saline (NS) using serum and liver extract at different concentrations during the period fr...
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ژورنال
عنوان ژورنال: Veterinary World
سال: 2011
ISSN: 0972-8988
DOI: 10.5455/vetworld.2011.503-506